Dr. Zzz Mercer University WebPage

 

Dr. Z        

 

                                                                                                                                                                 

                   

Luminal Cystine Transporter in the Proximal Tubule                                                   Metallothionein II   

 

A Little History of Dr. Z:

I was born of Latvian parents in a small farming town in the Georgian  bay region of Ontario, Canada.  I grew up in upstate New York during the 1960s and 1970s. I attended the State University of New York (SUNY) College at Brockport as an undergraduate and received a B.S. in Mathematics and Electronic Music (graduated Magna Cum Laude). I also received a M.S. in Zoology from SUNY Brockport (with a 4.0 GPA). Later, I received my doctorate in human anatomy and cell biology at the University of Western Ontario in London, Ontario, Canada (with a GPA of 4.0). I continued my training as a fellow and instructor at the Mayo Clinic, Yale University School of Medicine, University of Maryland School of Medicine and the University of Rochester School of Medicine and Dentistry. Later, I migrated to Macon, GA, where I joined the faculty of a newly formed medical school, Mercer University School of Medicine (MUSM). Currently, I am a tenured full professor at MUSM.

Research Interests:

My principle interests are in the area of the cellular and molecular biology and toxicology of select heavy metals, especially mercury, cadmium and uranium. Much of my work has focused on mechanisms by which the ionic species of mercury and cadmium gain entry into the primary target cells affected adversely (by these metals) in the kidneys, namely the epithelial cells lining the S1, S2 and S3 segments of the proximal tubule. Additionally, work in my lab has focused on functional significance of important sulfhydryl (SH)-containing cellular amino acids, peptides and proteins (such as cyteine (Cys), glutathione (GSH), and metallothionein (MT-1 and MT-2) that bind ionic species of mercury and cadmium. Moreover, my laboratory is interested in how these metal ions caninfluence the regulation of transcriptional and translation events relevant to the assembly of GSH and MT, as well as other SH-containing molecules.

Another pursuit in my laboratory is to gain a deeper understanding of the mechanisms involved in compensatory renal growth following significant reductions of renal mass (especially in states of renal disease). As a result of early work in my lab, we discovered a heterozygous, genetic mutant mouse that is born with the condition, Oligosyndactyly (allowing one to identify the mutant without doing PCR and/or genomic analyses). Intriguingly, these animals are derived from both inbreeding and outbreeding experiments at the Oak Ridge National Laboratory following irradiation experiments dating back to the 1950's during the Manhattan Project. Intriguingly, these mutant mice who express Oligosyndactyly, are born with 50% fewer nephrons than their normal liter mates. Furthermore, glomerulosclerosis develops in one strain of the mice. Accordingly, rediscovery of these animals has led to numerous important findings on compensatory renal growth (hypertrophy) and important insights into mechanisms of glomerulosclerosis in renal disease and mechanisms involved in nephrogenesis.

Below are a list of some of my published works. Articles depicted in red are linked to a "pdf"-version of the manuscript. Simply Left-Click on the mouse to activate the download sequence.

 

Peer-Reviewed Manuscripts Published:

1. Zalups RK, Knox FG. (1983). Calcitonin decreases the renal tubular capacity for phosphate reabsorption. Am J Physiol  24:F345-F348.

2. Zalups RK, Haase P, Philbrick DJ. (1983). Phosphate and the development of nephrocalcinosis in rats fed diets containing alpha protein. Am J Pathol  113:95-106.

3. Zalups RK, Haase P. (1983). The effects of parathyroidectomy on the development of nephrocalcinosis in rats fed phosphate-supplemented and unsupplemented diets containing alpha protein. Am J Pathol  113:107-111.

4. Zalups RK.(1984). Age and the development of nephrocalcinosis induced by injections of neutral phosphate in the rat. Acta Morphologica  32:121-125.

5. Zalups RK. (1984). Nephrocalcinosis and diets containing alkali- and non-alkali-treated soyprotein. Exp Mol Pathol  41:218-226.

6. Zalups RK. (1984). Influence of diet on the development of nephrocalcinosis in the rat following injections of neutral phosphate. Virchows Arch [Cell Pathol]  47:271-279.

7. Zalups RK, Stanton BA, Wade JB, Giebisch G. (1985). Structural adaptation in cortical collecting tubules following reduction of renal mass. Kidney Int  27:636-642.

8. Zalups RK, Diamond GL. (1987). Intrarenal distribution of mercury in the rat: Effect of administered dose of mercuric chloride. Bull Environ Cont Toxicol  38:67-72.

9. Zalups RK, Klotzbach JM, Diamond GL. (1987). Enhanced accumulation of injected inorganic mercury in renal outer medulla after unilateral nephrectomy. Toxicol Appl Pharmacol  89:226-236.

10. Zalups RK, Diamond GL. (1987). Mercuric chloride-induced nephrotoxicity in the rat following unilateral nephrectomy and compensatory renal growth. Virchows Arch B [Cell Pathol]  53:336-346.

11. Zalups RK, Sheu S-S. (1987). Effects of forskolin on intracellular sodium activity in resting and stimulated cardiac Purkinje fibers from sheep. J Mol Cell Cardiol  19:887-896.

12. Zalups RK, Diamond GL, Cox C. (1988). Histological and urinalysis assessment of nephrotoxicity induced by mercuric chloride in normal and uninephrectomized rats. In: Biological Monitoring of Toxic Metals. Ed. TW Clarkson, L Friberg, G Norberg, P Sager. Plenum Press New York. pp 531-545.

13. Zalups RK, Gelien RM, Morrow PE, Diamond GL. (1988). Uranyl fluoride-induced nephrotoxicity in uninephrectomized and sham-operated rats. Toxicol Appl Pharmacol  94:11-22.

14.Zalups RK, Veltman JC. (1988). Renal glutathione homeostasis in compensatory renal growth. Life Sci 42:2171-2176.

15. Zalups RK. (1989). Effect of dietary K+ and 75% nephrectomy on the morphology of principal cells in CCDs. Am J Physiol  256:F387-396.

16. Zalups RK, Lash LH. (1990). Effects of uninephrectomy and mercuric chloride on renal glutathione homeostasis. J Pharmacol Exp Ther  254:962-970.

17. Zalups RK, Barfuss D. (1990). Accumulation of inorganic mercury along the renal proximal tubule of the rabbit. Toxicol Appl Pharmacol  106:245-253.

18. Barfuss DW, Robinson MK, Zalups RK. (1990). Inorganic mercury transport in the proximal tubule of the rabbit. J Am Soc Nephrol  1:910-917.

19. Zalups RK. (1991). Autometallographic localization of inorganic mercury in the kidneys of rats: Effect of unilateral nephrectomy and compensatory renal growth. Exp Mol Pathol 54:10-21.

20. Zalups RK, Gelein, RM, Cernichiari E. (1991). DMPS as a rescue agent for the nephropathy induced by mercuric chloride. J Pharmacol Exp Ther  256:1-10.

21. Zalups RK. (1991). Renal accumulation and intrarenal distribution of inorganic mercury in the rabbit: Effect of unilateral nephrectomy and dose of mercuric chloride. J Toxicol Environ Health 33:213-228.

22. Zalups RK, Robinson MK, Barfuss DW. (1991). Factors affecting inorganic mercury transport and toxicity in the isolated perfused proximal tubule. J Am Soc Nephrol  2:866-878.

23. Zalups RK. (1991). Method for studying the in vivo accumulation of inorganic mercury in segments of the nephron in kidneys of rats treated with mercuric chloride. J Pharmacol Meth 26:89-104.

24. Zalups RK. (1991). An evaluation of tiron (sodium 4,5-dihydrobenzene-1,3-disulfonate) as a rescue agent for the nephropathy induced by uranyl fluoride (UO2F2) in rats. Res Commun Chem Pathol Pharmacol  72:125-128.

25. Zalups RK, Cherian G. (1992). Renal metallothionein metabolism after a reduction of renal mass. I. Effect of unilateral nephrectomy and compensatory renal growth on basal and metal-induced renal metallothionein metabolism. Toxicology  71:83-102.

26. Zalups RK, Cherian G. (1992). Renal metallothionein metabolism after a reduction of renal mass. II. Effect of zinc pretreatment on the renal toxicity and intrarenal accumulation of inorganic mercury. Toxicology  71:103-117.

27. Lash LH, Zalups RK. (1992). Mercuric chloride-induced cytotoxicity and compensatory hypertrophy in rat kidney proximal tubular cells. J Pharmacol Exp Ther  261:819-829.

28. Zalups RK, Barfuss DW, Kostyniak PJ. (1992). Altered intrarenal accumualtion of mercury in uninephrectomized rats treated with methylmercury  chloride. Toxicol Appl Pharmacol 115:174-182.

29. Zalups RK, Gelein RM, Cherian MG. (1992). Shifts in the dose-effect relationship for the nephropathy induced by cadmium-metallothionein in rats after a reduction in renal mass. J Pharmacol Exp Ther. 262:1256-1266.

30. Chan HM, Satoh M, Zalups RK, Cherian MG. (1992). Exogenous metallothionein and renal toxicity of cadmium and mercury in rats. Toxicology 76: 15-26.

31. Zalups RK, Henderson DM. (1992). Morphology of principal and intercalated cells in the outer medullary collecting duct of the rat: Effect of a 75% reduction of renal mass and potassium-depletion. Am J Physiol 263: F1119-F1127.

32. Zalups RK. (1993). Early aspects of the intrarenal distribution of mercury after the intravenous administration of mercuric chloride. Toxicology 79: 215-228.

33. Zalups RK. (1993). The Os/+ mouse: A genetic animal model of reduced renal mass. Am J Physiol. 264: F53-F60.

34. Robinson MK, Barfuss DW, Zalups RK. (1993). Cadmium transport and toxicity in isolated perfused segments of the renal proximal tubule. Toxicol Appl Pharmacol 121: 103-111.

35. Zalups RK, Barfuss DW. (1993). Transport and toxicity of methylmercury along the proximal tubule of the rabbit. Toxicol Appl Pharmacol 121: 176-185.

36. Zalups RK, Knutson KL, Schnellmann. (1993). In vitro analysis of the accumulation and toxicity of inorganic mercury in segments of the proximal tubule isolated from the rabbit kidney. Toxicol Appl Pharmacol 119: 221-227.

37. Zalups RK, MG Cherian, DW Barfuss. (1993). Mercury-metallothionein and the renal accumulation and handling of mercury. Toxicology. 83: 61-78.

38. Zalups RK. (1993). Intrarenal distribution of inorganic mercury and albumin after co-administration. J Toxicol Environ Health 40: 77-103.

39. Zalups RK. (1993). Influence of 2,3-dimercaptopropane-1-sulfonate (DMPS) and meso-2,3-dimercaptosuccinic acid (DMSA) on the renal disposition of mercury in normal and uninephrectomized rats exposed to inorganic mercury.  J Pharmacol Exp Ther 267: 791-800.

40. Lash LH, Zalups RK. (1994). Activities of enzymes involved in renal cellular glutathione metabolism after uninephrectomy. Arch Biochem Biophys 309: 129-138.

41. Zalups RK, Lash LH. (1994). Recent advances in the study of transport and toxicity of mercury in the kidney. J Toxicol Environ Health 42: 1-44.

42. Zalups RK, Cherian MG, Barfuss DW. (1995). Lack of luminal or basolateral uptake and transepithelial transport of mercury in isolated perfused proximal tubules exposed to mercury-metallothionein. J Toxicol Environ Health 44: 101-113.

43. Zalups RK (1995). Progressive losses of renal mass and the renal and hepatic disposition of administered inorganic mercury. Toxicol Appl Pharmacol  130: 121-131.

44. Zalups RK, Barfuss DW (1995). Accumulation and handling of inorganic mercury in the kidney after co-administration with glutathione. J Toxicol Environ Health 44: 385-399.

45. Zalups RK, Barfuss DW (1995). Renal disposition of mercury in rats after intravenous injection of inorganic mercury and cysteine. J Toxicol Environ Health 44: 401-413.

46. Zalups RK, Frazier J, Koropatnick J. (1995). Enhanced transcription of metallothionein genes in the kidney of the rat: Effect of uninephrectomy and compensatory renal growth.  Am J Physiol  268: F643-F650.

48. Zalups RK. (1995). Organic anion transport and action of g-glutamyltranspeptidase in the kidney linked mechanistically to renal tubular uptake of inorganic mercury. Toxicol Appl Pharmacol 132: 289-298.

49. Cijiang He,  Zalups RK, Henderson DA, Striker GE, Striker LJ. (1995). Molecular analysis of spontaneous glomerulosclerosis in Os/+ mice: A model with reduced nephron mass. Am J Physiol. 38: F266-F273.

50. Zalups RK, Barfuss DW. (1995). Pretreatment with p-Aminohippurate inhibits renal uptake and accumulation of  injected inorganic mercury. Toxicology. 103: 23-35.

51. Zalups RK, Minor KH. (1995). Luminal and basolateral mechanisms involved in the renal tubular uptake of inorganic mercury. J Toxicol Environ Health. 46: 73-100.

52. Striker GE, He C-J, Liu Z-H, Yang DCW, Zalups RK, Esposito C, Striker LJ. (1995). Biology of Disease: Pathogenesis of nonimmune glomerulosclerosis: Studies in animals and potential applications to humans. Lab Invest. 73: 596-605.

53. Lash LH, Zalups RK. (1996). Alterations in renal cellular glutathione metabolism after in vivo administration of a subtoxic dose of mercuric chloride. J Biochem Toxicol. 11: 1-9.

54. Zalups RK, Barfuss DW. (1996). Nephrotoxicity of inorganic mercury coadministered with L-cysteine.  Toxicology. 109: 15-29.

55. Ci-Jiang H, Esposito C, Phillips C, Zalups RK, Henderson DA, Striker GE, Striker LJ. (1996) Dissociation of glomerulosclerosis, glomerular hypertrophy and cell proliferation in mouse strains heterzygous for a mutation (Os) which induces a 50% reduction in nephron number. J Clin Invest 97: 1242-1249.

56. Zalups RK, Barfuss DW. (1996). Diversion or prevention of biliary outflow from the liver diminishes the renal uptake of injected inorganic mercury. Drug Metab Dispos. 24: 480-486.

57. Koropatnick J, Zalups RK. (1996). Effect of non-toxic mercury, zinc, or cadmium pre-treatment on the capacity of human monocytes to undergo lipopolysacharide-induced activation. Br J Pharmacol  120: 797-806.

58. Zalups RK. (1996). Enhanced renal outer medullary uptake of mercury associated with uninephrectomy: Implication of a luminal mechanism. J Toxicol Environ Health  50: 173-194.

59. Zalups RK. (1997). Reductions in renal mass and the nephropathy induced by mercury. Toxicol Appl Pharmacol. 143: 366-379.
 
60. Zalups RK, Lash LH. (1997). Binding of mercury in renal brush-border and basolateral membrane-vesicles: Implication of a cysteine-conjugate of mercury involved in the luminal uptake of inorganic mercury in the kidney. Biochem Pharmacol. 53: 1889-1900.

61. Zalups RK. (1997). Influence of different degrees of reduced renal mass on the renal and hepatic disposition of administered cadmium. J Toxicol Environ Health. 51: 245-264.

62. Zalups RK. (1997). Depletion of glutathione in the kidney and the renal disposition of administered inorganic mercury.  Drug Metab Dispos 25: 516-523.

63. Zalups RK, Barfuss DW. (1998). Small aliphatic dicarboxylic acids inhibit renal uptake of administered mercury. Toxicol Appl Pharmacol. 148: 183-193.

64. Zalups RK, Barfuss DW. (1998). Participation of mercuric conjugates of cysteine, homocysteine and N-acetylcysteine in mechanisms involved in the renal tubular uptake of inorganic mercury. J Am Soc Nephrol. 9: 551-561.

65. Zalups RK.(1998). Intestinal handling of mercury in the rat: Implication of intestinal secretion of inorganic mercury following biliary ligation or cannulation. J Toxicol Environ Health. 53: 615-636.

66. Diamond GL, Zalups RK. (1998). Understanding renal toxicity of heavy metals. J Toxicol Pathol. 26: 92-103.

67. Lash LH, Putt, DA Zalups RK. (1998). Role of extracellular thiols in uptake and distribution of inorganic mercury in rat renal proximal and distal tubular cells. J Pharmacol Exp Ther. 285: 1039-1050

68. Parks LD, Zalups RK, Barfuss DW (1998). Heterogeneity of glutathione synthesis and secretion in the proximal tubule of the rabbit. Am J Physiol. 274: F924-F931.

69. Zalups RK, Parks LD, Cannon VT, Barfuss DW (1998). Mechanisms of action of 1,2-dimercaptopropane-1-sulfonate and the transport, disposition and toxicity of inorganic mercury in isolated perfused segments of the rabbit proximal tubule. Mol Pharmacol 54: 353-363.

70. Zalups RK (1998). Basolateral uptake of mercuric conjugates of N-acteylcysteine and cysteine in the kidney involves the organic anion transport system. J Toxicol Environ Health. 55: 13-29

71. Zalups RK (1998). Basolateral uptake of inorganic mercury in the kidney. Toxicol Appl Pharmacol. 151:192-199.

72. Zalups RK, Barfuss DW, Lash LH (1999). Disposition of inorganic mercury following biliary obstruction and chemically-induced glutathione depletion: Dispositional changes one hour after the intravenous admimistration of mercuric chloride. Toxicol Appl Pharmacol. 154: 135-144.

73. Espisito C, He C-J, Striker GE, Zalups RK, Phillips C, Striker LJ (1999). The nature and severity of the glomerular response to nephron reduction is strain-dependent in mice. Am J Pathol. 154: 891-897.

74. Zalups RK, Barfuss DW, Lash LH (1999). Relationships between alterations in glutathione metabolism and the disposition of inorganic mercury: Effects of Biliary obstruction and chemically-induced modulation of glutathione status. Chem-Biol Interact 123: 171-195.

75. Lash LH, Putt, DA, Zalups RK. (1999). Influence of exogenous thiols on inorganic mercury-induced injury in renal proximal and distal tubular cells from normal and uninephrectomized rats. J Pharmacol Exp Ther. 291: 492-502.

76. Zalups RK (2000). Molecular interactions with mercury in the kidney. Pharmacol Rev 52: 113-143.

77. Zalups RK (2000). Evidence for basolateral uptake of cadmium in the kidneys of rats. Toxicol Appl Pharmacol. 164: 15-23.

78. Cannon VT, Barfuss DW, Zalups RK (2000). Molecular homology (“mimicry”) and the mechanisms involved in the luminal uptake of inorganic mercury in the proximal tubule of the rabbit. J Am Soc Nephrol 11: 394-402.

79. Parks LD, Zalups RK, Barfuss DW (2000). Luminal and basolateral membrane transport of glutathione in isolated perfused S1, S2, and S3 segments of the rabbit proximal tubule. J Am Soc Nephrol. 11: 1008-1015.

80. Zalups RK, Koropatnick J (2000). Temporal changes in metallothionein gene transcription in rat kidney and liver: Relationship to content of mercury and metallothionein protein. J Pharmacol Exp Ther. 295: 74-82.

81. Lash LH, Putt, DA, Zalups RK. (2000). Biochemical and functional characteristics of cultured renal epithelial cells from uninephrectomized rats: Factors influencing nephrotoxicity. J Pharmacol Exp Ther. 296: 243-251.

82. Lash LH, Putt, DA, Horky SJ (III), Zalups RK. (2001). Functional and toxicological characteristics of isolated renal mitochondria: Impact of compensatory renal growth. Biochem Pharmacol. 62: 383-395.

83. Cannon VT, Zalups RK, Barfuss DW. (2001). Amino acid transporters involved in the luminal transport of mercuric conjugates of cysteine in the rabbit proximal tubule. J Pharmacol Exp Ther. 298: 780-789.

84. Zalups RK, Barfuss DW. (2002). Simultaneous co-exposure to inorganic mercury and cadmium and its effects on the disposition of Mercury and Cadmium in the kidneys and liver. J Toxicol Env Health, 65:1471-1490.

85. Zalups RK, Barfuss DW. (2002). Renal organic anion transport system: A mechanism for the basolateral uptake of mercury-thiol conjugates along the pars recta of the proximal tubule. Toxicol Appl Pharmacol 182, 234-243.

86. Aslamkhan AG, Han Y-H, Zalups RK, Pritchard JB. (2003). Human renal organic anion transporter 1 (hOAT1) dependent uptake and toxicity of mercuric SH-conjugates in MDCK cells. Mol Pharmacol, 63, 590-596.

87. Zalups RK, Ahmad S. (2003). Molecular handling of cadmium in transporting epithelia. Toxicol Appl Pharmacol 186, 163-188.

88. Bridges CC, Bauch C, Verrey F, Zalups RK. (2004). Mercuric conjugates of cysteine are transported by the amino acid transporter, system b0,+: Implications of molecular mimicry. J Am Soc Nephrol. 15, 663-673.  

89. Zalups RK, Aslamkhan AG, Ahmad S. (2004). Biological Forms of Hg2+ are taken up by organic anion transporters through molecular homology. Kidney Int. 66, 251-261.

90. Butcher HL, Wendy A. Kennette WA, Collins O, Zalups RK, Koropatnick J. (2004). Metallothionein mediates the level and activity of nuclear factor kappa B (NFkB) in murine fibroblasts. J Pharmacol Exp Ther. 310, 589- 598.

91. Zalups RK, Ahmad S. (2004). Homocysteine and the renal epithelial transport and toxicity of inorganic mercury: Role of basolateral transporter OAT1 J Am So Nephrol. 15, 2023-2031.

92. Bridges CC, Zalups RK. (2004). Homocysteine, system b0,+, and the renal epithelial transport and toxicity of inorganic mercury. Am J Pathol 165, 1385-1394.

93. Bridges CC, Zalups RK. (2005). Molecular mimicry in metal ion transport. Tox Appl Pharmacol. 204, 274-308.

94. Bridges CC, Zalups RK. (2005). Cystine and glutamate transport in renal epithelial cells transfected with human system xc-. Kidney Int. 68, 653-664.

95. Zalups RK, Ahmad S. (2005). Handling of cysteine S-conjugates of methylmercury in MDCK cells expressing human OAT1.  Kidney Int. 68,1684-1699.

96. Zalups RK, Ahmad S. (2005). Transport of N-acetylcysteine S-conjugates of methylmercury in MDCK cells transfected stably with hOAT1. J. Pharmacol. Exp. Ther. 314:1158-1168.

97. Zalups RK, Ahmad, S. (2005). Handling of the Homocysteine S-Conjugate of Methylmercury by Renal Epithelial Cells: Role of OAT1 and Amino Acid Transporters. J Pharmacol Exp Ther. 315:896-904.

98. Kennette W, Collins OM, Zalups RK, Koropatnick J. (2005). Basal and zinc-induced metallothionein in resistance to cadmium, cisplatin, zinc, and tert-butyl hydroperoxide: Studies using MT knockout and antisense-downregulated MT in mammalian cell. Toxicol Sci. 88:602-613.

99. Lash LH, Hueni SE, Putt DA, Zalups RK. (2005). Role of organic anion and amino acid carriers in transport of inorganic mercury in rat renal basolateral membrane vesicles: Influence of compensatory renal growth. Toxicol Sci. 88:630-644.

100. Zalups RK, Lash LH. (2006). Cystine alters the renal and hepatic disposition of inorganic mercury and plasma thiol status. Toxicol Appl Pharmacol. 214: 88-97.

101. Bridges CC, Zalups RK. (2006). System B0,+ and the transport of thiol-S-conjugates of methylmercury. J Pharmacol Exp Ther. 319:948-956.

102. Lash LH, Putt DA, Zalups RK. (2006). Influence of Compensatory Renal Growth on Susceptibility of Primary Cultures of Renal Cells to Chemically Induced Injury. Toxicol Sci. Toxicol Sci. 94:417-427.

103. Zalups RK, Koropatnick J, Joshee L. (2007). Mouse Monocytes (RAW cells) and the Handling of Cysteine and Homocysteine S-Conjugates of Inorganic Mercury and Methylmercury. J Toxicol Environ Health.70:799-809.

104. Bridges CC, Joshee L, Zalups RK.. (2008). Multidrug resistance proteins and the renal elimination of inorganic mercury mediated by DMPS or DMSA. J Pharmacol Exp Ther. 324:383-90.

105. Bridges CC, Joshee L, Zalups RK..(2008). MRP2 and the DMPS- and DMSA-mediated elimination of mercury in TR(-) and control rats exposed to thiol S-conjugates of inorganic mercury. Toxicol Sci 105:211-220.

106. Zalups RK, Bridges CC. (2009). MRP2 involment in renal tubular eliminaiton of methylmercury mediated by DMSA or DMPS. Toxicol Appl Pharmacol. 235:10-17.

107. Bridges CC, Joshee L, Zalups RK.. (2009). Effect of DMPS and DMSA on the placental and fetal disposition of methylmercury. Placenta 30: 800-8005.

108. Ekstrand J, Nielsen JB, Havarinasab S, Zalups RK, Söderkvist P, Hultman P. (2009). Mercury toxicokinetics-dependency on strain and gender. Toxicol Appl Pharmacol. 243: 283-291.

109. Wang Y, Barfuss DW, Zalups RK. (2010). Potential mechanisms involved in the absorptive transport of cadmium in isolate perfused rabbit renal proximal tubules. Toxicol Lett. 193:61-68.

110. Bridges CC, Zalups RK. (2010). Transport of inorganic mercury and methylmercury in target tissues and organs. J Toxicol Environ Health B Crit Rev.13:385-410.

111. Wang Y, Zalups RK, Barfuss DW. (2010). Potential mechanisms involved in the absorptive transport of cadmium in isolated perfused rabbit renal proximal tubules. Toxicol Lett. 193:61-68.

112. Zalups RK, Bridges CC. (2010). Seventy-five percent nephrectomy and the disposition of inorganic mercury in 2,3-dimercaptopropanesulfonic acid-treated rats lacking functional multidrug-resistance protein 2. J Pharmacol Exp Ther.332:866-75.

113. Bridges CC, Joshee L, Zalups RK. (2011). MRP2 and the handling of mercuric ions in rats exposed acutely to inorganic and organic species of mercury. Toxicol Appl Pharmacol 251:50-58.

114. Wang Y, Zalups RK, Barfuss DW. (2012). Luminal transport of thiol S-conjugates of methylmercury in isolated perfused rabbit renal proximal tubules. Toxicol Lett. 213:203-210.

115. Bridges CC, Joshee L, Zalups RK. (2012) Placental and fetal disposition of mercuric ions in rats exposed to methylmercury: role of Mrp2. Reprod Toxicol. 34:628-634.

116. Zalups RK, Bridges CC.(2012) Relationships between the renal handling of DMPS and DMSA and the renal handling of mercury. Chem Res Toxicol. Sep 25:1825-1838.

117. Bridges CC, Krasnikov BF, Joshee L, Pinto JT, Hallen A, Li J, Zalups RK, Cooper AJ. (2012). New insights into the metabolism of organomercury compounds: mercury-containing cysteine S-conjugates are substrates of human glutamine transaminase K and potent inactivators of cystathionine γ-lyase. Arch Biochem Biophys. 517:20-29.

118. Bridges CC, Joshee L, van den Heuvel JJ, Russel FG, Zalups RK. (2013). Glutathione status and the renal elimination of inorganic mercury in the Mrp2(-/-) mouse. PLoS One. 8(9):e73559.

119. Bridges CC, Joshee L, Zalups RK. (2014). Aging and the disposition and toxicity of mercury in rats. Exp Gerontol. 53:31-39.

120. Zalups RK, Joshee L, Bridges CC. (2014). Novel Hg2+-induced nephropathy in rats and mice lacking Mrp2: evidence of axial heterogeneity in the handling of Hg2+ along the proximal tubule. Toxicol Sci.142:250-260.

 

PEER-REVIEWED BOOKS PUBLISHED:

 

INVITED REVIEWS:

1. Zalups RK, Lash LH. (1994). Recent advances in the study of transport and toxicity of mercury in the kidney. J Toxicol Environ Health 42: 1-44.

2. Zalups RK (2000). Molecular interactions with mercury in the kidney. Pharmacol Rev. 52: 113-143.

3. Zalups RK, Ahmad S. (2003). Molecular handling of cadmium in transporting epithelia. Toxicol Appl Pharmacol 186, 163-188.

4. Bridges CC, Zalups RK.(2005). Molecular mimicry in metal ion transport. Toxicol Appl Pharmacol. 204, 274-308.

5. Zalups RK, Bridges CC (2012). Relationships between the renal handling of DMPS and DMSA and the renal Handling of Mercury. Chem Res Toxicol. 204, 274-308.

 

 INVITED CHAPTERS IN BOOKS:

1. Zalups RK, Lash LH. (1996). Interactions between glutathione and mercury in the kidney, liver and blood. In Toxicology of Metals. LW Chang, T Suzuki, L Magos (Eds). Chapter 9, CRC Press. Boca Raton. pp. 145-164

2. Zalups RK, Barfuss DW. (1996a). The isolated perfused tubule as a technique to study the transport and toxicity of metals along the nephron. In Toxicology of Metals. LW Chang, T Suzuki, L Magos (Eds).Chapter 4, CRC Press, Boca Raton. pp. 765-782

3. Zalups RK, Barfuss DW. (1996b). In Vitro Perfusion of Isolated Nephron Segments: A Method for Renal Toxiciolgy. In Methods in Renal Toxicology. RK Zalups, LH Lash (Eds).Chapter 8, CRC Press,  Boca Raton. pp. 123-146

4. Zalups RK.(1997). Renal toxicity of mercury. In Comprehensive Toxicology. IG Sipes, CA McQueen, AJ Gandolfi (Eds) Volume 7, Renal Toxicology. RS Goldstein (section Ed.). Chapter 33, pp. 633-652. Elsevier Science LTD, Amsterdam. pp. 633-651.

5. Koropatnick J, Suzzane D, Zalups RK. (1999). Metallothionein and the activation of primary human and mouse monocytes. In Trace Elements in Man and Animals. AM Roussel, RA Anderson, and AV Favrier, eds. Kluwer Academic/Plenum Publishers, New York. pp. 47-50.

6. Zalups RK. (2000). Mercury: Molecular interactions and mimicry in the kidney. In Molecular Biology and Toxicology of Metals. RK Zalups and DJ Koropatnick (Eds). Chapter 9, Taylor & Francis, London. pp. 234-237.

7. Koropatnick DJ, Zalups RK (2000). Toxic and essential metals in the cellulsar response to signals.In Molecular Biology and Toxicology of Metals. RK Zalups and DJ Koropatnick (Eds). Chapter 18. Taylor and Fancis, London. pp. 551-576

8. Zalups RK, Diamond GL. (2005). Nephrotoxicology of Metals. In Toxicology of the kidney. JB Tarloff and LH Lash (Eds). Chapter 22. CRC Press, Boca Raton, pp. 937-993.

9. Berlin M, Zalups RK, Fowler BA. (2007). Mercury. In Handbook of the Toxicology of Metals. GF Nordberg, BA Fowler, M Nordberg, LT Friberg (Eds). Chapter 33. Elsevier-Academic Press, Amsterdam, pp.633-652.

10. Zalups RK. (2010). Renal handling and toxicity of mercury. In Comprehensive Toxicology. Second Edition, IG Sipes, CA McQueen, AJ Gandolfi (Eds). Volume 7, Renal Toxicology, RS Goldstein (Ed), Chapter 33, Elsevier Science LTD, Amsterdam, pp. 633-652.

11. Bridges CC, Zalups RK, (2010). Ionic and Molecular Mimicry and the transport of metals. In Cellular and Molecular Biology of Metals. RK Zalups and DJ Koropatnick (Eds). Chapter 10. CRC Press, Boca Raton. pp. 241-294.

12. Zalups RK, Bridges CC (2010). Molecular and cellular biology of mercury in the kidneys. In Cellular and Molecular Biology of Metals. RK Zalups and DJ Koropatnick (Eds). Chapter 10. CRC Press, Boca Raton. pp. 35-77.

13. Bridges CC, Zalups RK, (2013). The aging kidney and exposure to the nephrotoxic metals cadmium and mercury. In Aging and Vulnerability to Environmental Chemicals: Age-Related Disorders and Their Origins in Environmental Exposures. Issue in Toxicology. No. Royal Society of Chemistry. pp. 336-364.

14. Zalups RK, Bridges CC (2016). Renal toxicity of mercury. In Comprehensive Toxicology. IG Sipes, CA McQueen, AJ Gandolfi (Eds) Volume 7, Renal Toxicology. RS Goldstein (section Ed.). Chapter 33, pp. 633-652. Elsevier Science LTD, Amsterdam. pp. 633-651

 

RESEARCH SUPPORT:

2011-2014      “Proximal Tubular Transport of Mercury and Effects of Reduced Renal Mass.” Principal Investigator: Grant Awarded by National Institute of Environmental Health Sciences. Grant Number: 1 R15 ES019991-01. Christy C. Bridges, Co-Investigator: Rudolfs K. Zalups. Total Awarded: $471,000.

2007-2009     “Role of Multidrug Resistance Proteins 2 and/or 4 in Renal Elimination of Mercury” Grant Awarded by National Institute of Environmental Health Sciences. Grant Number: RO3 ES015511. Principal Investigator: Christy C. Bridges, Co-Investigator: Rudolfs K. Zalups. Total Amount Awarded = $146,200

2002-2010     "Transport and Toxicity of Mercury in the Nephron". Grant renewed by National  Institute of Environmental Health Science. Grant Number: RO1 ES05980 Principal Investigator: Rudolfs K. Zalups. This grant received a 1 percentile ranking, on a first submission to the ALTX 4 Study Section. Total Amount Awarded = $1,324,220.

2002-2009     Administrative supplement for “"Transport and Toxicity of Mercury in the Nephron". National Institute of Environmental Health Science. Grant Number: RO1 ES05980 Principal Investigator: Rudolfs K. Zalups. Total Amount Awarded = $125,000 ($25,000 per annum for the manufacture of 203Hg++).

2002-2009     "Metals, Metallothionein and Monocyte Activation". New Grant. National Instiute of Environmental Health Science. Grant  Number: RO1 ES05157. Principal Investigator: Rudolfs K. Zalups, Ph.D., This grant received a 6 percentile ranking, on a first submission to the ALTX 1 Study Section. Total Amount Awarded = $1,562,000.

2000-2004       "Mercury Nephrotoxicity After a Reduction of Renal Mass".  Grant Renewed. National Institute of Environmental Health Science. Grant Number: RO1 ES05157. Principal Investigator: Rudolfs K. Zalups, Ph.D.  Total Amount Awarded = $750,000.

1998-2003     "Transport and Toxicity of Mercury in the Nephron". Grant renewed by National  Institute of Environmental Health Science. Grant Number: 1 RO1 ES05980 Principal Investigator: Rudolfs K. Zalups. Total Amount Awarded = $1,179,271.

1998-1999     Grant Award from the Fraternal Order of Eagles. Principal Investigator: Rudolfs K. Zalups, Ph.D. Total Amount Awared = $5,000.

1996-2000     Supplement for under represented minorities. "Mercury Nephrotoxicity After a Reduction of Renal Mass".  This supplement was to support graduate student Vernon T. Cannon. Grant Number: ES05157-S. Principal Investigator: Rudolfs K. Zalups, Ph.D. Total Amount Awarded = $50,000

1994-1998     "Mercury Nephrotoxicity After a Reduction of Renal Mass".  Grant Awarded by the National Instiute of Environmental Health Science. Grant  Number: RO1 ES05157. Principal Investigator: Rudolfs K. Zalups, Ph.D., Total Amount Awarded = $810,000.

1993-1997     "Transport and Toxicity of Mercury in the Nephron". Grant awarded by National  Institute of Environmental Health Science. Grant Number: 1 RO1 ES05980 Total Award = $818,947. Principal Investigator: Delon W. Barfuss, Co-Investigator: Rudolfs K. Zalups. Total Costs Awarded to Co-PI = $435,350.

1993-1994     An Additional Year of Funding has been Awarded for the Grant "Mercury Nephrotoxicity After a Reduction of Renal Mass" by the National Institute of Environmental Health Science. Grant Number ES 05157-6. Principal Investigator = Rudolfs K. Zalups. The estimated Direct Costs for this Additional Year of Funding = $40,000.           

1992-1993     "Transport and Toxicity of Mercury in the Nephron". Shannon Award Granted from 9/1/92 - 2/1/92 by National Institutes of Health. Grant Number: 1 R55 ES05980 Total Award = $50,000. Principal Investigator: Delon W. Barfuss, Co-Invesitigator: Rudolfs K. Zalups. Total amount awarded to Co-PI = $25,000.

1990-1991      NIH Supplement Award. Grant Awarded by the National Institute of Environmental Health Science. Award date: 6/90.. Principal Investigator: Rudolfs K. Zalups, Ph.D. Total award for the period 6/90 - 5/91 = $7,500.

1989-1990     Small Instrumentation Grant. Grant Awarded by the National Institute of Environmental Health Science. Grant Number: 1 S15 ES05288. Award Date: 5/89. Principal Investigator: Rudolfs K. Zalups, Ph.D. Total Award = $5,339.

1988-1993     "Mercury Nephrotoxicity After a Reduction of Renal Mass". National Institutes of Health First Award. Grant awarded by the National Institute of Environmental Health Science.Grant Number: 1 R29 ES05157. Award Date: 9/88.. Principal Investigator: Rudolfs K. Zalups, Ph.D. Total Award = $415,800.

1986-1987       "Effect of Unilateral Nephrectomy on the Nephropathy Induced by Mercuric Chloride". Pilot Project Grant. Awarded from the Center Grant given to the Division of Toxicology at the University of Rochester Medical Center by NIH. Principal Investigator: Rudolfs K.Zalups, Ph.D. Total Award = $6,000.

1985-1986       "Genesis of Calcium-Induced Arrhythmogenic Current in the Heart", Grant Awarded by the American Heart Association, Genesee Valley Affiliate, New York. Principal Investigator: Rudolfs K. Zalups, Ph.D., Total Award = $25,000.

1984-1985       "Digitalis-Induced Arrhythmogenic Current in Heart". Grant Awarded by the American Heart Association, Genesee Valley Affiliate, New York. Principal Investigator: Rudolfs K. Zalups, Ph.D., Total Award = $9,000.

 

HONORS AND AWARDS:

1985-86           American Heart Association Fellowship Award.

1984-85           American Heart Association Fellowship Award.

1979-81           University Graduate Student Scholarship, University of Western Ontario.

1981-82          First Recipient of the C.P. Leblond Research Presentation Award (Awarded a Graduate Student in a Canadian Department of Anatomy and/or Cell Biology by the Canadian Anatomical Association).

1982                Mayo Clinic Fellowship Award (HIH Training Grant).

1998-99           First Recipient of The Mercer University Award for Excellence in Research, for the most outstanding research carried out by a faculty member across all colleges at Mercer University.

1998                Research Award from Fraternal Order of Eagles.

2002                Nominated for Merit Award by NIEHS for NIH Grant ES05980

 

REVIEWER FOR PEER-REVIEWED JOURNALS:

                        American Journal of Physiology

                        American Journal of Pathology

                        Archives of Environmental Contamination and Toxicology

                        Arteriosclerosis, Thrombosis, and Vascular Biology

                        Biochemica et Biophysica Acta

                        Biochemical Pharmacology

                        Cellular and Molecular Biology

                        Chemico-Biological Interactions

                       Chemistry and Biology

Chemical & Engineering News

                        Comparative Biochemistry and Physiology

                        Chemical Research in Toxicology

                        Drug Metabolism and Disposition

                        Environmental Health Perspectives

                        International Immunopharmacology

                        Kidney International

                        Life Science

                        Neuro-Toxicology

                        Journal for the American Society of Nephrology

                        Journal of Histochemistry and Cytochemistry

                        Journal of Pharmacology and Experimental Therapeutics

                        Journal of Toxicology Environmental Health

                        Pharmacological Research

                        Proceedings for the Society of Experimental Biology and Medicine

                        Toxicology and Applied Pharmacology

                        Toxicology

                        Toxicology Letters

                        Toxicological Sciences

 

MEMBERSHIP TO EDITORIAL BOARDS:

                        Toxicology and Applied Pharmacology:  1999 - 2003


                        Journal of Toxicology Environmental Health: 1994 - present

 

ASSOCIATE EDITOR:
                                   
                        Journal of Toxicology Environmental Health: 1994 - Present

 

SOCIETY MEMBERSHIPS:

American Physiological Society

American Society of Nephrology (1988-1990)

American Society of Pharmacology and Experimental Therapeutics

Society of Toxicology

 

 

CD Histology

                            Epithelium                                                                                    Glands 

                                   Muscle                                                                          Cartilage and Bone                        

Instructions: To view the content of any of the above histology modules, simply download the linked executable file onto your computer (only with Windows operating systems). To download the associated exe-file, simply Left-Click on any of the Images to download the executable file for the Epithelium, Glands, Muscle, or Cartilage and Bone module. After downloading the file, double-click on the executable file to launch the respective histology module.

 

Free Music of Dr. Z

After years of composing contemporary and electronic music, I decided that it was time to allow others free access to some of my more favorite pieces. Therefore,..... help yourselves to any or all the works I have provided on this site. I would appreciate opinions from any or all. There is one condition to the use of my copywritten music files ........................................................... PROFESSIONAL AND COMMERCIAL USE OF ANY MUSICAL FILES BY DR Z. IS STRICTLY PROHIBITED!!!!!! If one would like to use any of my works for commercial purposes, please contact me at the following email address:   zalups_rk@mercer.edu.

All works provided below were composed, arranged, produced, performed, engineered, and recorded by Dr. Z. Most of the works provided below were composed about 15-20 years ago. I will be gearing up to get back into composition and performance in the near future. Each musical file is in 128 bit-mp3 format, which is CD quality. To download a song, just right click over the highlighted song title and then choose the "save target as" option from the pop-up menu. Use a good pair of headphones and/or a good stereo system inorder to appreciate the spacial aspects of each song. Moreover, please play these musical works at Flat EQ, because all of the songs have been mastered to target 1db at flat equalization; adding bass or treble may result in audio distortion.

Finally, HOPE YOU ENJOY!!

Musical Works of Dr. Z                                                                                                                          

Alien Talk, Alien Talk Revisted, Away from Home, Areospase, B3, Big Bang, Bump on a Log, Buzz-Bomb, Buzzed, Buzzy, Carlos, Cosmo Girl, Crying Alone, Crying Solo, Dancing on the Floor of Life, Dark Cave, Darkened Sky, Deep, Dreamy Guitar, Dig-Beat, Distortion, Dream Weaver, Echo, Forest, Gameland, Happy Day, Hard Drive, Hip-Hop Jazz, Hip-Hop Screams, Hopping Along to Eternity, IMP, Interlude, Italia, Jazz Trip, Lead Me, Leading, Meander, Melody, Miami Nights, My Dream, Out at Night, Outland, Phat Guitar, Returning Home, Sawtooth,Singing, Slowly, Smooth, Spanish Fly, Step Up, Strange Thoughs, Straty, Sweet-Breath, Synth-Licks, Traveling Afar, Trumpity-Sax, Tsunami, Walking at Night, Walking Away, Walking Alone, Whales, Wide Open Spaces, Winter Wind, Wishes, With Open Arms.

 

Musical Videos byDr. Z:

Edited and/or generated by Dr. Z. Some of the music in these videos is composed and performed by Dr. Z and represents work carried out some 20-25 years ago.

 

Haunting Feeling on a Fall Morning on the Lake of the Woods

 

I Can't Drive 55

 

Red Flag Exercise at Nellis Air Force Base

 

Primates Feeding

 

Lions

 

Panda